Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (P13L, Δ31-33, E136D, R203K, G204R, S413R) , partial

• CAS Number:

  9000-83-3

• Gene Name:

  N

• UniProt:

  P0DTC9

• Expression Region:

  1-419aa (P13L, Δ31-33, P151S, R203K, G204R, S413R)

• Organism:

  Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

• Target Sequence:

  MSDNGPQNQRNAPRITFGGPSDSTGSNQNGGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATDGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSRADSTQA

• Tag:

  N-terminal 6xHis-tagged

• Source:

  E.coli

• Field of Research:

  Microbiology

• Assay Type:

  Developed Protein

• Relevance:

  Essential role in cell cycle control. PP2A may be involved in controlling the entry into mitosis, possibly acting as an inhibitor.

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Length:

  Partial

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  49.5 kDa

• References & Citations:

  "Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe." Kim D.U., Hayles J., Kim D., Wood V., Park H.O., Won M., Yoo H.S., Duhig T., Nam M., Palmer G., Han S., Jeffery L., Baek S.T., Lee H., Shim Y.S., Lee M., Kim L., Heo K.S. Hoe K.L. Nat Biotechnol 28:617-623 (2010)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.