Recombinant Kukulcania hibernalis Omega-filistatoxin-Kh1a

• Product Name Alternative:

  Omega-FLTX-Kh1a; Toxin DW13.3

• Abbreviation:

  Recombinant Kukulcania hibernalis Omega-filistatoxin-Kh1a protein

• UniProt:

  P60979

• Expression Region:

  1-74aa

• Organism:

  Kukulcania hibernalis (Southern house spider) (Filistata hibernalis)

• Target Sequence:

  AECLMIGDTSCVPRLGRRCCYGAWCYCDQQLSCRRVGRKRECGWVEVNCKCGWSWSQRIDDWRADYSCKCPEDQ

• Tag:

  C-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Potently blocks vertebrate calcium channels Cav1 and Cav2. Is the most active on Cav2.2/CACNA1B (from HEK) (IC (50) =2.3 nM), followed by Cav2.1/CACNA1A (IC (50) =4.3 nM), Cav2.2/CACNA1B (from oocyte) (IC (50) =14.4 nM), Cav1.2/CACNA1C (IC (50) =26.8 nM), and Cav2.3/CACNA1E (IC (50) =96.4 nM) .

• Endotoxin:

  Not test

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  15.6 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length