Recombinant Human Collagen alpha-1 (XV) chain (COL15A1), partial

• Product Name Alternative:

  Collagen alpha-1 (XV) chain [Cleaved into: Restin (Endostatin-XV) (Related to endostatin) (Restin-I) ; Restin-2 (Restin-II) ; Restin-3 (Restin-III) ; Restin-4 (Restin-IV) ]

• Abbreviation:

  Recombinant Human COL15A1 protein, partial

• Gene Name:

  COL15A1

• UniProt:

  P39059

• Expression Region:

  40-228aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  HLDLTQLIGVPLPSSVSFVTGYGGFPAYSFGPGANVGRPARTLIPSTFFRDFAISVVVKPSSTRGGVLFAITDAFQKVIYLGLRLSGVEDGHQRIILYYTEPGSHVSQEAAAFSVPVMTHRWNRFAMIVQGEEVTLLVNCEEHSRIPFQRSSQALAFESSAGIFMGNAGATGLERFTGSLQQLTVHPDP

• Tag:

  N-terminal 10xHis-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Cancer

• Relevance:

  Structural protein that stabilizes microvessels and muscle cells, both in heart and in skeletal muscle.; Restin potently inhibits angiogenesis.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  26.5 kDa

• References & Citations:

  "Antiangiogenic activity of restin, NC10 domain of human collagen XV: comparison to endostatin." Ramchandran R., Dhanabal M., Volk R., Waterman M.J.F., Segal M., Lu H., Knebelmann B., Sukhatme V.P. Biochem. Biophys. Res. Commun. 255:735-739 (1999)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial