Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Propionate kinase (tdcD)

• Abbreviation:

  Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B tdcD protein

• Gene Name:

  TdcD

• UniProt:

  A1JIM9

• Expression Region:

  1-406aa

• Organism:

  Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

• Target Sequence:

  MTLNPTVLVINCGSSSIKFSVLTADNCEAVISGIADGIGTEKPFLRIDRVTQFQLAKWNYSDALAAIADELDKRGLSKSISLIGHRIAHGGEIFSESVLIDDRVVEEIKKVSPLAPLHNYANLHGVGAARQLFPGIKQVAVFDTGFHQTLKPEAYLYALPYRYFREQGVRRYGFHGTSYRYVVGEAASFLGFDGSDCGLIIAHLGNGASLCAVQDGKSVDTSMGMTPLEGLIMGTRSGDVDYGALAYLARQTGQSLEDLDHMVNKESGLLGISGLSADMRVLENAYHEGHEGARLAINTFVHRLARHIGGHASSLRRFDALIFTGGIGENSSLIRQLTLEHLAVFGIDIDHAKNKRLQHGTSQIITTARSRVTAAVIPTNEEKMIALDAIRLGYAQQGTVVLEAVI

• Tag:

  C-terminal 6xHis-tagged

• Type:

  In Stock Protein

• Source:

  Baculovirus

• Field of Research:

  Others

• Relevance:

  Catalyzes the conversion of propionyl phosphate and ADP to propionate and ATP.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  49.6 kDa

• References & Citations:

  "The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081." Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. Prentice M.B. PLoS Genet. 2:2039-2051 (2006)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length