Recombinant Human Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A), partial (Active)

• Product Name Alternative:

  Tumor necrosis factor receptor superfamily member 10A; Death receptor 4; TNF-related apoptosis-inducing ligand receptor 1; TRAIL receptor 1; TRAIL-R1; CD antigen CD261

• Abbreviation:

  Recombinant Human TNFRSF10A protein, partial (Active)

• Gene Name:

  TNFRSF10A

• UniProt:

  O00220

• Expression Region:

  24-239aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  ASGTEAAAATPSKVWGSSAGRIEPRGGGRGALPTSMGQHGPSARARAGRAPGPRPAREASPRLRVHKTFKFVVVGVLLQVVPSSAATIKLHDQSIGTQQWEHSPLGELCPPGSHRSEHPGACNRCTEGVGYTNASNNLFACLPCTACKSDEEERSPCTTTRNTACQCKPGTFRNDNSAEMCRKCSRGCPRGMVKVKDCTPWSDIECVHKESGNGHN

• Tag:

  C-terminal 10xHis-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Cancer

• Relevance:

  Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:26457518, PubMed:38532423) . The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (PubMed:19090789) . Promotes the activation of NF-kappa-B (PubMed:9430227) . {ECO:0000269|PubMed:19090789, ECO:0000269|PubMed:38532423, ECO:0000269|PubMed:9430227}.

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  Greater than 95% as determined by SDS-PAGE. Greater than 95% as determined by SEC-HPLC.

• Activity:

  Yes

• Bioactivity:

  1Measured by its binding ability in a functional ELISA.Immobilized Human TNFRSF10A at 2 μg/mL can bind anti-TNFRSF10A recombinant antibody (CSB-RA023964MA1HU) .The EC50 is 1.038-1.260 ng/mL. 2TNFRSF10A Recombinant Monoclonal Antibody (CSB-RA023964MA1HU) captured on Protein A Chip can bind Human TNFRSF10A with an affinity constant of 0.851 nM as detected by MetaSPR Assay (WeSPRTM 200) .

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  24.2 kDa

• References & Citations:

  Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Sugano S. Nat. Genet. 36:40-45 (2004)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Transmembrane Domains:

  0TM

• Protein Length:

  Partial