Recombinant Human Girdin (CCDC88A), partial

• Product Name Alternative:

  (Akt phosphorylation enhancer) (APE) (Coiled-coil domain-containing protein 88A) (G alpha-interacting vesicle-associated protein) (GIV) (Girders of actin filament) (Hook-related protein 1) (HkRP1)

• Abbreviation:

  Recombinant Human CCDC88A protein, partial

• Gene Name:

  CCDC88A

• UniProt:

  Q3V6T2

• Expression Region:

  1713-1823aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  SLSVSSDFLGKDKPVSCGLARSVSGKTPGDFYDRRTTKPEFLRPGPRKTEDTYFISSAGKPTPGTQGKIKLVKESSLSRQSKDSNPYATLPRASSVISTAEGTTRRTSIHD

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Neuroscience

• Relevance:

  Bifunctional modulator of guanine nucleotide-binding proteins (G proteins) . Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G (i) alpha subunits . Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G (s) subunit alpha GNAS . Essential for cell migration . Interacts in complex with G (i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration . Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex which enhances phosphoinositide 3-kinase (PI3K) -dependent phosphorylation and kinase activity of AKT1/PKB . Phosphorylation of AKT1/PKB induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation . Binds in its tyrosine-phosphorylated form to the phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1 which enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration . Plays a role as a key modulator of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation . Inhibition of G (s) subunit alpha GNAS leads to reduced cellular levels of cAMP and suppression of cell proliferation . Essential for the integrity of the actin cytoskeleton . Required for formation of actin stress fibers and lamellipodia . May be involved in membrane sorting in the early endosome . Plays a role in ciliogenesis and cilium morphology and positioning and this may partly be through regulation of the localization of scaffolding protein CROCC/Rootletin .

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  16.1 kDa

• References & Citations:

  "GIV/Girdin activates Galphai and inhibits Galphas via the same motif." Gupta V., Bhandari D., Leyme A., Aznar N., Midde K.K., Lo I.C., Ear J., Niesman I., Lopez-Sanchez I., Blanco-Canosa J.B., von Zastrow M., Garcia-Marcos M., Farquhar M.G., Ghosh P. Proc. Natl. Acad. Sci. U.S.A. 113:E5721-E5730 (2016)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial