Recombinant Pig Transferrin receptor protein 1 (TFRC), partial

• Product Name Alternative:

  TR; TfR; TfR1; Trfr; CD71

• Abbreviation:

  Recombinant Pig TFRC protein, partial

• Gene Name:

  TFRC

• UniProt:

  Q8HZV3

• Expression Region:

  90-768aa

• Organism:

  Sus scrofa (Pig)

• Target Sequence:

  AYCKRVESKTDCKTLVPTEPSETEETETFEAENFPQTPRLFWADLKILLSKGLDTTDFTRTIKMLNEDYAPREAGSQKDESLGFFIENQFREFKLSKVWHDEHFVKIQVKGSNAENSVTLVNTDSNSLVYPVESPEGYVAYSKATTVTGKLIFANFGTKKDFEDLKMPVNGSLVIVRAGKITFAEKVANAQSLDAIGVLIYMDRANFPIINADVPVFGHAHLGTGDPYTPGFPSFNHTQFPPSQSSGLPNIPVQTISRAGAEKLFGNMEQDCPLTWRTDFPCKLVSSPSKNVKLTVNNVLKEIKILNIFGVIKGFEEPDRYVIVGAQRDAWGPGAAKSSVGTSLLLNLAQILSDMVIKGQFKPSRSIVFASWSAGDFGAIGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYSLIEKMMQDVKNPVTGQSLYRDSNWINKVEKLSFDDAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYDVLSKRVPQLNRMARAAAEVAGHLVIKLTIDFELNLNYEMYNDKILSFVREMNQFRVDIREMGLSLQWLYSARGDFFRATSRLTSDYRNVETRDKFVMREINDRIMKVEYHFLSPYVSPRESPFRHIFWGSGSHTLSALVEHLKLRQKNSSAFNQTLLKNQLALATWTIQGAANALSGDIWDIDNEF

• Tag:

  N-terminal 10xHis-tagged

• Type:

  Developed Protein

• Source:

  Mammalian cell

• Field of Research:

  Cancer

• Relevance:

  Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2. Mediates uptake of NICOL1 into fibroblasts where it may regulate extracellular matrix production

• Endotoxin:

  Not test

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Not test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  79.9 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial