Recombinant Mesocricetus auratus Programmed Cell death 1 ligand 1 isoform X1 (Cd274), partial, Biotinylated

• Product Name Alternative:

  B7 homolog 1

• Abbreviation:

  Recombinant Mesocricetus auratus Cd274 protein, partial, Biotinylated

• Gene Name:

  Cd274

• UniProt:

  A0A1U7Q5Z5

• Expression Region:

  20-238aa

• Organism:

  Mesocricetus auratus (Golden hamster)

• Target Sequence:

  FTITAPKELYEVEYGSNVTIECRFPVKHQLDLLSLVVYWEKNDNPVIQFVDGKVDLKHQHSSFRGRAWLPKEQILKGNAGLQITNVKLQDAGVYGCMISYGGADYKRITLQVNAPYRKINHRISMDPVTSEHELTCQAEGYPEAEVIWTNSDKQSLSDKTTVTISQTEEKLFNVTSTLRINATANDAFYCTFWRLQSGENHTAELIIPEPLAEFLPNKR

• Tag:

  C-terminal GS-hFc1-Avi-tagged

• Type:

  Developed Protein

• Source:

  Mammalian cell

• Field of Research:

  Others

• Relevance:

  The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy

• Endotoxin:

  Not test

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  53.0 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial