Recombinant Bacteroides gingivalis Major fimbrial subunit protein type-2 (fimA)

• Product Name Alternative:

  Fimbrillin; Major fimbrial subunit protein type II

• Abbreviation:

  Recombinant Porphyromonas gingivalis fimA protein

• Gene Name:

  FimA

• UniProt:

  Q51825

• Expression Region:

  47-386aa

• Organism:

  Porphyromonas gingivalis (Bacteroides gingivalis)

• Target Sequence:

  AFGNAGDEAKVAKLTVMVYKGEQQEAIKSAENATKVENIKCSAGQRTLVVMANTGGMELAGKTLAEVKALTTELTEGNQEAAGLIMTAEPVEVTLVAGNNYYGYDGSQGGNQISQDTPLEIKRVRARIAFTKIEVTMSQSYANKYNFAPENIYALVAKKKSNLFGASLANNDDAYLTGSLTTFNGAYTPANYTHVDWLGRDFTEPSNNAPQGFYVLESTYAQNAGLRPTILCIKGKLTKHDGTPLSSEEMTAAFNAGWIVANNDPTTYYPVLVNFESNNYTYTGEAVEKGKIVRNHKFDINLTITGPGTNNPENPITESANLNVNCVVAAWKGVVQNVIW

• Tag:

  C-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Structural subunit of the major fimbriae (Probable) . These long, filamentous pili are attached to the cell surface; they mediate biofilm formation, adhesion onto host cells and onto other bacteria that are part of the oral microbiome. They play an important role in the invasion of periodontal tissues. Fimbriae and their constituents are major virulence factors. FimA proteins from different strains have highly divergent sequences, and this has been used for classification. The sequence-based classification correlates with pathogenicity

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  43.7 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein