Recombinant Mouse Tribbles homolog 3 (Trib3)

• Product Name Alternative:

  (TRB-3) (Neuronal cell death-inducible putative kinase)

• Abbreviation:

  Recombinant Mouse Trib3 protein

• Gene Name:

  Trib3

• UniProt:

  Q8K4K2

• Expression Region:

  1-354aa

• Organism:

  Mus musculus (Mouse)

• Target Sequence:

  MRATPLAASADVSCRKKPLEFDDNIDAKCPVLKRVRDEPEPGPLPSLLPPSPPPASDLSPAVAPATRLGPYILLEREQGSCSYRALHCPTGTEYTCKVYPASEAQAVLAPYARLPTHQHVARPTEVLLGSRLLYIFFTKTHGDLHSLVRSRRGIPESEAAGLFRQMASAVAHCHKHGLVLRDLKLRRFVFSNCERTKLVLENLEDACVMTGSDDSLWDKHACPAYVGPEILSSRPSYSGKAADVWSLGVALFTMLAGRYPFHDSEPVLLFGKIRRGTFALPEGLSAPARCLIRCLLRKEPSERLVALGILLHPWLREDHGRVSPPQSDRREMDQVVPDGPQLEEAEEGEVGLYG

• Tag:

  N-terminal 10xHis-tagged and C-terminal Myc-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Cardiovascular

• Relevance:

  Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress . Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress . May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells . Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity . Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation . May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 . Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity . Interacts with MAPK kinases and regulates activation of MAP kinases . Can inhibit APOBEC3A editing of nuclear DNA .

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  46.5 kDa

• References & Citations:

  "TRB3 inhibits the transcriptional activation of stress-regulated genes by a negative feedback on the ATF4 pathway." Jousse C., Deval C., Maurin A.C., Parry L., Cherasse Y., Chaveroux C., Lefloch R., Lenormand P., Bruhat A., Fafournoux P. J. Biol. Chem. 282:15851-15861 (2007)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length