Recombinant Halobacterium salinarum Cobalamin-binding protein (btuF)

• Product Name Alternative:

  Vitamin B12-binding protein

• Abbreviation:

  Recombinant Halobacterium salinarum btuF protein

• Gene Name:

  BtuF

• UniProt:

  B0R5G2

• Expression Region:

  25-369aa

• Organism:

  Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)

• Target Sequence:

  LAPQPPAQHADADRACSFPVTEPDASNTAITLDSEPERVVTLNPSAAQTMWELGDRDAVVGVSQFGTYLPTASQRTVVSGGQPSQTNVEAVVGLDPDLVLAPNTVRNTTVTRLRSAGITVFQFRAATSIDGVVEKTATIGRLTGNCAAAAATTAEMRDRVAAIADAVPDTDSARPRVYYHLGDGYTAGPNTFIGAAIEAAGGHNIAADVNTTSSYPQLSEEVIVSQDPDVVVTGVSADRLDATASALVAPSSVVRNTTAYATGNVVAVNTNHINQPAPRIVEPMARMANAFHNTTINTTLDAQPSATTTATSTAPPTDAADGTAPGFGVAAAVCALAGAALVARR

• Tag:

  Tag-Free

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin import. Probably binds cobalamin and delivers it to the surface of BtuC.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably binds cobalamin and delivers it to the surface of BtuC (By similarity) .

• Molecular Weight:

  35.3 kDa

• References & Citations:

  "Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346 (2008)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein