Recombinant Larimichthys crocea Hepcidin (hamp)

• Product Name Alternative:

  Hamp; Hepcidin

• Abbreviation:

  Recombinant Larimichthys crocea hamp protein

• Gene Name:

  Hamp

• UniProt:

  A1Z0M0

• Expression Region:

  65-85aa

• Organism:

  Larimichthys crocea (Large yellow croaker) (Pseudosciaena crocea)

• Target Sequence:

  RCRFCCRCCPRMRGCGICCRF

• Tag:

  Tag-Free

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Immunology

• Relevance:

  Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages Has very strong antibacterial activity against the marine Gram-negative bacteria V.alginolyticus (MIC=24 µM), V.fluvialis, V.harveyis (MIC=12 µM) and V.parahaemolyticus (MIC=6 µM) . Has antibacterial activity against the Gram-negative bacteria A.hydrophila (MIC=6 µM), E.coli (MIC=24 µM), and E.coli BL21 (DE3) plysS (MIC=6 µM), and the Gram-positive bacteria B.cereus (MIC=24 µM), B.subtilis (MIC=6 µM), C.glutamicum (MIC=3 µM), M.luteus (MIC=3 µM), M.lysodeikticus, S.aureus (MIC=6 µM) and S.epidermis (MIC=12 µM) . Possesses antifungal activity against A.niger (MIC=24 µM), F.graminearum (MIC24 µM) and F.solani (MIC=24 µM), but lacks antifungal activity against the yeasts P.pastoris GS115 and C.albicans.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages (By similarity) .

• Molecular Weight:

  2.5 kDa

• References & Citations:

  "Isolation and characterization of a hepcidin peptide from the head kidney of large yellow croaker, Pseudosciaena crocea." Zhang J., Yan Q., Ji R., Zou W., Guo G. Fish Shellfish Immunol. 26:864-870 (2009)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein