Recombinant Sulfolobus islandicus Chromatin protein Cren7 (creN7)

• Product Name Alternative:

  CreN7; LS215_1335Chromatin protein Cren7

• Abbreviation:

  Recombinant Sulfolobus islandicus creN7 protein

• Gene Name:

  CreN7

• UniProt:

  C3MPN0

• Expression Region:

  1-60aa

• Organism:

  Sulfolobus islandicus (strain L.S.2.15 / Lassen #1)

• Target Sequence:

  MSSGKKAVKVKTPAGKEAELVPEKVWALAPKGRKGVKIGLFKDPETGKYFRHKLPDDYPI

• Tag:

  N-terminal GST-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  A probable chromatin protein, binds double-strand DNA without sequence specificity. Constrains negative DNA supercoils.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  A probable chromatin protein, binds double-strand DNA without sequence specificity. Constrains negative DNA supercoils.

• Molecular Weight:

  33.6 kDa

• References & Citations:

  Biogeography of the Sulfolobus islandicus pan-genome.Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J.Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610 (2009)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length