Recombinant Rabbit Melanotransferrin (Mfi2), partial

• Product Name Alternative:

  Membrane-bound transferrin-like protein p97; CD228

• Abbreviation:

  Recombinant Rabbit Mfi2 protein, partial

• Gene Name:

  Mfi2

• UniProt:

  O97490

• Expression Region:

  20-710aa

• Organism:

  Oryctolagus cuniculus (Rabbit)

• Target Sequence:

  SVEVRWCTASEPEQQKCEDMSQAFREAGLQPALLCVQGTSADHCVQLIAAHEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVKRSSNVTINTLRGVKSCHTGINRTVGWNVPVGYLVDSGRLSVMGCDVLKAVSEYFGGSCVPGAGETRYSESLCRLCRGDTSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGRTLPSWGHMLMSRDFELLCRDGSRASVTEWQHCHLARVPAHAVVVRADTDAGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKNLLFKDSTLELVPIATQTYEAWLGPEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFSRQRLKPEIQCVSAESPQHCMEQIQAGHIDAVTLNGEDIHTAGKTYGLIPAAGELYAADDRSNSYFVVAVVKRDSAYAFTVDELRGKRSCHPGFGSPAGWDVPVGALIHWGYIRPRNCDVLTAVGQFFNASCVPVNNPKKYPSSLCALCVGDEQGRNKCTGNSQERYYGDSGAFRCLVEGAGDVAFVKHTTIFDNTNGHNPEPWAAHLRSQDYELLCPNGARAEAHQFAACNLAQIPSHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSSYHGRDLLFKDATVRAVPVGERTTYQDWLGPDYVAALEGMQSQRCS

• Tag:

  N-terminal 6xHis-SUMO-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Involved in iron cellular uptake. Ses to be internalized and then recycled back to the cell mbrane. Binds a single atom of iron per subunit. Could also bind zinc .

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc (By similarity) .

• Molecular Weight:

  91.7 kDa

• References & Citations:

  Expression of membrane-bound transferrin-like protein p97 on the cell surface of chondrocyte.Kawamoto T., Pan H., Yan W., Ishida H., Usui E., Oda R., Nakamasu K., Noshiro M., Kawashima-Ohya Y., Fujii M., Shintani H., Okada Y., Kato Y.Eur. J. Biochem. 256:503-509 (1998)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial