The nucleocapsid protein (N) is a viral protein that binds to the viral RNA, playing a significant role in the packaging of the genome into new viral particles. It has key functions related to RNA-binding, replication, and virion assembly, making it a vital component of the virus's structure. Additionally, its high abundance and strong immunogenicity make it ideal for use in assays aimed at detecting and studying viral infections. ScienceDirecte

The nucleocapsid protein is often used as an antigen in a variety of research applications, including :

1. Antibody screening (for diagnostic tests).
2. Antigen detection assays (such as ELISA).
3. Protein interaction studies (e.g., immunoprecipitation).
4. Viral infection studies (for understanding host-pathogen interactions).

Not all nucleocapsid proteins are identical across different strains of viruses. Mutations and deletions within the protein can lead to changes in its structure and antigenicity. These variations can impact how the protein interacts with antibodies, and subsequently, how well assays using this protein perform. This makes it important to select the correct variant-specific nucleocapsid protein for your research, depending on the strain you are studying.

For instance, specific point mutations and deletions in the nucleocapsid protein can influence its interaction with antibodies, which is critical for ensuring that your assays provide accurate and consistent results. By using variant-specific proteins, researchers can ensure that their assays are aligned with the correct viral strain, improving the reliability and sensitivity of the diagnostic tools.

Karobio offers a high-quality recombinant nucleocapsid protein that is engineered to be used in ELISA, Western blot, and other antigen detection assays. The product includes specific variants of the nucleocapsid protein that have been identified as important in research applications involving viral infections.

-Product Name : Recombinant Nucleocapsid Protein

-Catalog Number : 399-GTR04821685

-Size Options : 100 µg

-Expression Host : Escherichia coli

-Purity : >85% (SDS‑PAGE)

-Molecular Weight : ~52.8 kDa

-Tags : N-terminal His-tag and C-terminal Myc-tag

-Storage : Available in liquid or lyophilized form (with recommended storage conditions)

This product is ideal for use in a range of applications, including antigen detection, antibody screening, and immunoassay development. The inclusion of His-tags ensures easy purification and handling, and the protein is available with high purity to guarantee accurate results in your assays.

-Variant-Specific Protein for Assay Accuracy : By choosing a variant-specific nucleocapsid protein, researchers can ensure their assays are aligned with the correct viral strain, improving the accuracy and reliability of their experiments. The protein is available with P13L, Δ31–33, R203K, and G204R mutations to match specific viral variants, which are important for accurate antibody testing and antigen detection.

-High Purity for Reliable Results : With purity greater than 85% as confirmed by SDS-PAGE, the protein is ideal for antigen detection assays and immunoassay calibration, where consistency and quality are crucial for reproducibility. The protein's high purity ensures minimal interference in your assay results, allowing you to obtain clear and precise data.

-Easy Handling with Affinity Tags : The inclusion of both His-tags and Myc-tags simplifies the purification process, making this recombinant protein easier to work with. Whether you're conducting antibody binding studies or protein-protein interaction assays, these tags provide a convenient way to monitor and purify the protein.

1) Antigen Detection Assays : Karobio's recombinant nucleocapsid protein is an excellent choice for ELISA-based assays. By coating the plates with the recombinant protein, researchers can develop assays that are highly sensitive to antibodies specific to the nucleocapsid protein, facilitating the detection of viral exposure or infection.

2) Western Blot and Immunoassay Controls : This product is ideal for use as a control protein in Western blot experiments, allowing researchers to verify antibody specificity and performance. The high purity of the protein ensures that the results are reproducible and reliable, making it a valuable asset in quality control and assay validation.

3) Antibody Screening and Validation : For those developing polyclonal or monoclonal antibodies, the recombinant nucleocapsid protein is an ideal tool for antibody screening. It provides a reliable antigen for testing antibody reactivity and ensuring that the antibodies produced will specifically bind to the nucleocapsid protein in various assays.

For researchers focused on viral diagnostics, antibody development, and antigen detection, Karobio's recombinant nucleocapsid protein is a versatile and high-performance tool that can enhance the accuracy and reliability of your assays. With specific variant sequences and high purity, this product is perfect for a range of applications from ELISA to Western blotting.

Visit Karobio's product page to purchase this high-quality protein and elevate your research today!